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Biol. 220 - Microbiology:  LABORATORY SCHEDULE

Date:

Exercise # (Microbiology: Laboratory Theory and Application)

Week 1 9/30-10/2       Lab. safety/safety sheets

Exercise: 1-2 (Aseptic technique)

Culture transfer technique

i.  Simple transfers

Exercise:  1-3 (Isolation of Colonies)

a.  Spread and streak plate technique

Exercise:  3-1, 3-3, 3-10 (Microscopy)

a.  General microscopy techniques

i.   care of microscopes

ii. Brightfield:  observe slides of different types of microbes

iii. Hanging drop:  Observe living material (motility of bacteria)

Week 2 10/7-9 Exercise:  3-4, 3-5, 3-6 (Staining techniques). 

Smear preparation and simple staining

Negative stain

Gram stain

Week 3 10/14-16 Exercise:  3-6, 3-7, 3-8, 3-9  (Staining cont.)

Gram stain unknowns

Acid-fast staining

Capsule staining

Endospore staining

Week 4 10/21-23        Exercise:  4-1, 4-5, 4-7, 4-8; 2-10, 2-11 (Properties of Microbes)

a.  Differential/Selective medias (Mannitol, Eosin-methylene blue,  MacConkey)

b.      Physical factors:  temperature and pH

Week 5 10/28-30 Biochemical Reactions (Differential tests):

Fermentation reactions:

a. Exercise 5-2: Carbohydrate fermentation

b. Exercise 5-21:  Triple sugar iron (TSI)

Enzyme hydrolysis

a. Exercise 5-13 (starch), 5-16 (casein), 5-17 (gelatin), 5-19 (lipid)

b. Exercise 5-5 (catalase)

IMViC

a. Exercise 5-20 (indole, H₂S), 5-4 (MR-VP), 5-8 (citrate)

Multi-test Strips

-30:  Enterotube II (Enterobacteriaceae)

Week 6 11/4-6            ***LAB PRACTICAL*** (1^st 4 weeks)

Identification of Pathogens

                                                a. Staphylococcal pathogens

i. 5-27 (coagulase)

                                                            ii. 5-18 (DNase)

                                                            iii. 4-2 (Mannitol agar)

                                                            iv. Handout  (Novobiocin susceptibility)

b. Streptococcal pathogens

i. 5-12 (bile esculin)

                                                            ii. 5-26 (blood agar)

                                                            iii. 5-24 (bacitracin sensitivity)

                                                            iv.  Handout  (6.5% NaCl)

Week 7 11/12-13*** No class Tuesday 11/11 – make up period 11/25 or by arrangement

Control of microbes

a. 7-3:  Kirby-Bauer antimicrobial susceptibility test

b. 8-5:  Methylene blue reductase

                                                c.  Disinfectant lab (handout)

                                    Microbial Genetics

                                                a. 6-4:  Plaque assay

                                                b.  Transformation (handout)

Week 8 11/18-20                   Transformation day 2 (handout)

Immunology

                                                a. 10-1:  Blood count

                                                b. 10-5:  Blood typing

                                                c.  Handout [Directigen kit (or SureView Strep kit)]

Eukaryotic Microbes

                                                a. 11-1:  Fungi

                                                b. 11-2: Protozoans

                                                c. 11-3:  Worms

Week 9           11/25               No lab – Thanksgiving week

Week 10  12/2-12/4    ***LAB PRACTICAL #2** (week 5 on)